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Localization of fixed dipole emitters

scientific illustration shows Point spread function of a fixed dipole emitter

© TU Wien

Point spread function of a fixed dipole emitter

The intensity peak is shifted with respect to the true position of the emitter.

Our paper “Robust and bias-free localization of individual fixed dipole emitters achieving the Cramer Rao bound for applications in cryo-single molecule localization microscopy”, in cooperation with the Johannes Kepler University Linz and the RICAM Institute of the ÖAW, was published in PLOS ONE. We show how the problem of localization bias for fixed dipole emitters can be circumvented by using an astigmatic imaging approach. Our approach allows for unbiased localization estimates even under defocused imaging conditions, while achieving the best possible localization precision at the Cramer-Rao bound.