Analytic Laboratory

Chromatography

© TU Wien

Chromatography, interior view of the laboratory

The laboratory of the institute is located on the second floor of the Wasserforschungslabor at TU Wien, Lilienthalgasse 21, Object OD.

The main tasks of the laboratory are the accompanying investigations of the projects worked on at the institute and to a small extent contract analysis. The laboratory carries out routine investigations of ground, surface and wastewater as well as analyses of gas and solid samples.

The laboratory is also equipped for special investigations and for the preparation of expert opinions. Due to the constantly increasing number of new projects, new demands are constantly being placed on the analysis in our laboratory. Therefore a permanent introduction of new analytical methods, development of new parameters and the use of modern high-tech equipment is necessary.

Laboratory equipment

Our laboratory consists of three areas of competence:

  • chemical-physical laboratory,
  • micro & molecular biology laboratory and
  • Research lab.

In this linked file you will find the extensive list of applied methods and investigated parameters:
Methods and Parameter (pdf), opens a file in a new window

Nutrient analysis

The nutrient analysis (e.g. NH4, NO3, NO2, TP, TN, TKN) is mainly carried out with the San++ Continuous-Flow Analyser from Skalar. Four different parameters (NH4, NO3, NO2 and PO4) are measured simultaneously from one sample using colorimetric methods.

The SAN++ software package controls the entire analyser and supports automatic calculation of results, reporting, statistical evaluation and quality control.

The oxidative digestion for the determination of total bound nitrogen (TN) or total bound phosphorus (TP) is performed by oxidative microwave digestion with an instrument from the company Berghof.

Determination of Kjeldahl nitrogen (TKN) and total phosphorus (TP) in particle-rich samples is performed with a Kjeldahl digestion block with 36 digits (up to 400°C) from Gerhardt. The subsequent colorimetric determination of ammonium and phosphorus is performed with the continuous flow analyser.

Analyzer nutrient analysis

© TU Wien

Analyzer nutrient analysis

Chart Nutrients

© TU Wien

Chart Nutrients

Devices for nutrient analysis

© TU Wien

Devices for nutrient analysis

Devices for nutrient analysis

© TU Wien

Devices for nutrient analysis

Carbon analysis

For carbon analysis, in addition to the wet chemical methods (COD) and the BOD determination, a TOC analyser from Shimadzu is used. The device works with catalytic combustion oxidation at 680°C and can be used for the analysis of ultrapure water as well as highly polluted wastewater.

The instrument is equipped with additional modules for the determination of total bound nitrogen by chemiluminescence detection and a module for the determination of carbon in solid samples (sludge, sediments). The control, evaluation and quality assurance is possible by a software package from Shimadzu.

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 TOC analyser from Shimadzu

© TU Wien

TOC analyser from Shimadzu

 TOC analyser from Shimadzu

© TU Wien

TOC analyser from Shimadzu

Aqualog spectral fluorimeter (Fa Horiba)

© TU Wien

Aqualog spectral fluorimeter (Fa Horiba)

Diagram fluorescence spectroscopy

© TU Wien

Diagram fluorescence spectroscopy

Fluorescence spectroscopy

Fluorescence spectroscopy examines the fluorescence of a sample when it is excited with UV light, visible light or light from the near infrared spectrum. Our laboratory is equipped with an Aqualog spectral fluorimeter (Fa Horiba), which simultaneously measures absorption spectra and fluorescence excitation emission matrices (EEM).

Ion chromatography

Hitachi ion chromatography systems are used to determine the various main inorganic ions in water and other ions such as bromide / bromate and organic acids.

Hitachi ion chromatography system

© TU Wien

Hitachi ion chromatography system

Analysis of organic trace substances

The impact of treated wastewater on the environment has been increasingly addressed in recent years. However, the analysis of standard parameters is not sufficient to describe the behaviour of various environmentally relevant substances such as pharmaceutically active substances, pesticides, hormones or even industrial and household chemicals.

For the investigation of environmentally relevant substances, which are often present in very low concentrations (ng/L), various sample preparation or chromatographic methods are used in the laboratory.

HPLC + Triple Quadrupole MS

The high-performance liquid chromatography (HPLC) system from Agilent in combination with a triple quadrupole mass spectrometer from the company AB Sciex (QTrap 6500+) with high detection sensitivity and selectivity is used for the quantitative and qualitative analysis of micropollutants.

High-performance liquid chromatography (HPLC) system from Agilent

© TU Wien

High-performance liquid chromatography (HPLC) system from Agilent

Chart high-performance liquid chromatography (HPLC) system

© TU Wien

Chart high-performance liquid chromatography (HPLC) system

GC

In addition to the determination of environmentally relevant substances such as OCP, PAH and nonylphenols, permanent gases (H2, CO2, N2O, O2, N2, CH4, CO) are analysed by gas chromatography.

The chromatographic separation of the samples is performed on a TRACE GC with split/splitless or PTV injector and Trace Autosampler, which is also equipped with headspace. Using different detectors such as TCD, ECD and a DSQ Single Quadrupole it is possible to determine a wide range of substances and to achieve low determination and detection limits.

chromatographic separation

© TU Wien

chromatographic separation

Molecular microbiology

Many of our projects also involve microbiological and molecular biological investigations. In addition to classical microbiological analyses, high-resolution microscopic methods and innovative genetic investigations are also carried out. For the enrichment of the microorganisms to be examined or their genetic material (DNA/RNA), equipment such as a filtration bar with connected vacuum pump and various high-speed refrigerated centrifuges are used. For the extraction and purification of DNA and RNA we also use a FastPrep-24™ 5G instrument from MP Biomedicals and for automated extraction a Maxwell® RSC instrument from Promega.
For the determination of bacterial density by turbidity measurement as well as for the concentration determination of nucleic acids and for the quality assessment of DNA/RNA extracts, we have a BioSpectrometer® fluorescence from Eppendorf at our disposal, with which both spectrophotometric and fluorimetric measurements can be performed. For the sensitive detection and quantification of the microbial target DNA/RNA present in a sample, our laboratory is equipped with both a quantitative and a digital PCR instrument. In addition to the Applied Biosystems QuantStudio™ 6 Pro Real-Time PCR System from ThermoFisher Scientific, we also have access to advanced dPCR technology with the Applied Biosystems QuantStudio Absolute Q Digital PCR System from ThermoFisher Scientific. For visualization of DNA/RNA fragments we use a gel documentation system from Nippon Genetics.
For microscopic analyses we have the confocal laser scanning microscope Stellaris 5 - Lightning from Leica. This is an inverted research microscope (DMi8 CS Premium) with a motorized scanning stage equipped with 4 high-power lasers for excitation in the UV range (405 nm) as well as in the blue (488 nm), green (561 nm) and red wavelength range (638 nm) and a high-resolution scanner (Stellaris 5) as well as 3 highly sensitive HyD detectors. In addition to classical light microscopic examinations, this high-end instrument also enables us to perform confocal microscopy, fluorescence microscopic methods and 3D imaging.

View into a lab room with boxes and equipment

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Molecular Biology Laboratory

Maxwell® RSC instrument incl. Quantus™ fluorometer from Promega for automated DNA/RNA extraction incl. DNA/RNA quantification & FastPrep-24™ 5G instrument from MP Biomedicals

© TU Wien

Maxwell® RSC instrument incl. Quantus™ fluorometer from Promega for automated DNA/RNA extraction incl. DNA/RNA quantification & FastPrep-24™ 5G instrument from MP Biomedicals

Applied Biosystems QuantStudio™ 6 Pro Real-Time PCR System from ThermoFisher Scientific

© TU Wien

Applied Biosystems QuantStudio™ 6 Pro Real-Time PCR System from ThermoFisher Scientific

Stellaris 5 - Lightning confocal laser scanning microscope from Leica

© TU Wien

Stellaris 5 - Lightning confocal laser scanning microscope from Leica

Online - measuring instruments

The monitoring of various chemical-physical parameters with on-line devices, both in wastewater treatment plants and in water bodies, is becoming increasingly important and has developed strongly over the last 20 years. The projects in this field are intensively supported by the laboratory and this enables the further development of methods and devices in cooperation with industrial partners.

Additional tasks

Administrative activities

A separate laboratory information system has been developed for administrative activities in the laboratory such as sample registration, archiving of measurement results and reporting. This facilitates the exchange of important data between the laboratory and its staff. A separate software has been developed for various methods such as oxygen consumption measurement of activated sludge.

Training and further education

In addition to analysis, the laboratory also has a training and further education function. On the one hand, new employees are to be familiarized with various analytical methods, on the other hand, interested students and trainees have the opportunity to receive further training in environmental analysis. Various training projects on a national and international (IAESTE, Erasmus etc.) level have been supported.

Contact

Water research laboratory

Head of Laboratories

Dipl.-Ing. Dr.techn.
Ernis Saracevic

Location Arsenal

Lilienthalgasse 21
A-1030 Vienna
Austria

E-Mail: erni@iwag.tuwien.ac.at
Phone: +43 1 58801 22660